Manganese Induces Oxidative Stress, Redox State Unbalance and Disrupts Membrane Bound ATPases on Murine Neuroblastoma Cells In Vitro: Protective Role of Silymarin.
Identifieur interne : 001484 ( Main/Exploration ); précédent : 001483; suivant : 001485Manganese Induces Oxidative Stress, Redox State Unbalance and Disrupts Membrane Bound ATPases on Murine Neuroblastoma Cells In Vitro: Protective Role of Silymarin.
Auteurs : Yassine Chtourou [Tunisie] ; Khaled Trabelsi [Tunisie] ; Hamadi Fetoui [Tunisie] ; Ghada Mkannez [Tunisie] ; Héla Kallel [Tunisie] ; Najiba Zeghal [Tunisie]Source :
- Neurochemical Research [ 0364-3190 ] ; 2011-04-30.
Descripteurs français
- Wicri :
- topic : Manganèse.
English descriptors
Abstract
Manganese (Mn) is an essential trace element required for ubiquitous enzymatic reactions. Chronic overexposure to this metal may promote potent neurotoxic effects. The mechanism of Mn toxicity is not well established, but several studies indicate that oxidative stress play major roles in the Mn-induced neurodegenerative processes. Silymarin (SIL) has antioxidant properties and stabilizes intracellular antioxidant defense systems. The aim of this study was to evaluate the toxic effects of MnCl(2) on the mouse neuroblastoma cell lines (Neuro-2A), to characterize the toxic mechanism associated with Mn exposure and to investigate whether SIL could efficiently protect against neurotoxicity induced by Mn. A significant increase in LDH release activity was observed in Neuro-2A cells associated with a significant decrease in cellular viability upon 24 h exposure to MnCl(2) at concentrations of 200 and 800 μM (P < 0.05) when compared with control unexposed cells. In addition, exposure cells to MnCl(2) (200 and 800 μM), increases oxidant biomarkers and alters enzymatic and non enzymatic antioxidant systems. SIL treatment significantly reduced the levels of LDH, nitric oxide, reactive oxygen species and the oxidants/antioxidants balance in Neuro-2A cells as compared to Mn-exposed cells. These results suggested that silymarin is a powerful antioxidant through a mechanism related to its antioxidant activity, able to interfere with radical-mediated cell death. SIL may be useful in diseases known to be aggravated by reactive oxygen species and in the development of novel treatments for neurodegenerative disorders such as Alzheimer or Parkinson diseases.
Url:
DOI: 10.1007/s11064-011-0483-5
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000659
- to stream Hal, to step Curation: 000659
- to stream Hal, to step Checkpoint: 000570
- to stream Main, to step Merge: 001554
- to stream Main, to step Curation: 001484
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Manganese Induces Oxidative Stress, Redox State Unbalance and Disrupts Membrane Bound ATPases on Murine Neuroblastoma Cells In Vitro: Protective Role of Silymarin.</title><author><name sortKey="Chtourou, Yassine" sort="Chtourou, Yassine" uniqKey="Chtourou Y" first="Yassine" last="Chtourou">Yassine Chtourou</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-161034" status="INCOMING"> <orgName>Animal Physiology Laboratory, Life Sciences Department</orgName> <desc> <address> <addrLine>UR/08-73, Sfax University, BP 1171, 3000 Sfax</addrLine> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-309870" type="direct"></relation> </listRelation><tutelles><tutelle active="#struct-309870" type="direct"><org type="institution" xml:id="struct-309870" status="INCOMING"> <orgName>Faculty of Sciences</orgName> <desc> <address> <country key="FR"></country> </address> </desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Trabelsi, Khaled" sort="Trabelsi, Khaled" uniqKey="Trabelsi K" first="Khaled" last="Trabelsi">Khaled Trabelsi</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-73827" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13 Place Pasteur, BP 74 1002 Tunis, Belvédère</addrLine><country key="TN"></country></address></desc><listRelation><relation active="#struct-300103" type="direct"></relation><relation active="#struct-301247" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine><country key="TN"></country></address><ref type="url">http://www.pasteur.tn/</ref></desc></org></tutelle><tutelle active="#struct-301247" type="direct"><org type="institution" xml:id="struct-301247" status="VALID"><orgName>Réseau International des Instituts Pasteur</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Fetoui, Hamadi" sort="Fetoui, Hamadi" uniqKey="Fetoui H" first="Hamadi" last="Fetoui">Hamadi Fetoui</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-161034" status="INCOMING"> <orgName>Animal Physiology Laboratory, Life Sciences Department</orgName> <desc> <address> <addrLine>UR/08-73, Sfax University, BP 1171, 3000 Sfax</addrLine> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-309870" type="direct"></relation> </listRelation><tutelles><tutelle active="#struct-309870" type="direct"><org type="institution" xml:id="struct-309870" status="INCOMING"> <orgName>Faculty of Sciences</orgName> <desc> <address> <country key="FR"></country> </address> </desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Mkannez, Ghada" sort="Mkannez, Ghada" uniqKey="Mkannez G" first="Ghada" last="Mkannez">Ghada Mkannez</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-73827" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13 Place Pasteur, BP 74 1002 Tunis, Belvédère</addrLine><country key="TN"></country></address></desc><listRelation><relation active="#struct-300103" type="direct"></relation><relation active="#struct-301247" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine><country key="TN"></country></address><ref type="url">http://www.pasteur.tn/</ref></desc></org></tutelle><tutelle active="#struct-301247" type="direct"><org type="institution" xml:id="struct-301247" status="VALID"><orgName>Réseau International des Instituts Pasteur</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Kallel, Hela" sort="Kallel, Hela" uniqKey="Kallel H" first="Héla" last="Kallel">Héla Kallel</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-73827" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13 Place Pasteur, BP 74 1002 Tunis, Belvédère</addrLine><country key="TN"></country></address></desc><listRelation><relation active="#struct-300103" type="direct"></relation><relation active="#struct-301247" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine><country key="TN"></country></address><ref type="url">http://www.pasteur.tn/</ref></desc></org></tutelle><tutelle active="#struct-301247" type="direct"><org type="institution" xml:id="struct-301247" status="VALID"><orgName>Réseau International des Instituts Pasteur</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Zeghal, Najiba" sort="Zeghal, Najiba" uniqKey="Zeghal N" first="Najiba" last="Zeghal">Najiba Zeghal</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-161034" status="INCOMING"> <orgName>Animal Physiology Laboratory, Life Sciences Department</orgName> <desc> <address> <addrLine>UR/08-73, Sfax University, BP 1171, 3000 Sfax</addrLine> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-309870" type="direct"></relation> </listRelation><tutelles><tutelle active="#struct-309870" type="direct"><org type="institution" xml:id="struct-309870" status="INCOMING"> <orgName>Faculty of Sciences</orgName> <desc> <address> <country key="FR"></country> </address> </desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:pasteur-00606405</idno><idno type="halId">pasteur-00606405</idno><idno type="halUri">https://hal-riip.archives-ouvertes.fr/pasteur-00606405</idno><idno type="url">https://hal-riip.archives-ouvertes.fr/pasteur-00606405</idno><idno type="doi">10.1007/s11064-011-0483-5</idno><date when="2011-04-30">2011-04-30</date><idno type="wicri:Area/Hal/Corpus">000659</idno><idno type="wicri:Area/Hal/Curation">000659</idno><idno type="wicri:Area/Hal/Checkpoint">000570</idno><idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000570</idno><idno type="wicri:doubleKey">0364-3190:2011:Chtourou Y:manganese:induces:oxidative</idno><idno type="wicri:Area/Main/Merge">001554</idno><idno type="wicri:Area/Main/Curation">001484</idno><idno type="wicri:Area/Main/Exploration">001484</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Manganese Induces Oxidative Stress, Redox State Unbalance and Disrupts Membrane Bound ATPases on Murine Neuroblastoma Cells In Vitro: Protective Role of Silymarin.</title><author><name sortKey="Chtourou, Yassine" sort="Chtourou, Yassine" uniqKey="Chtourou Y" first="Yassine" last="Chtourou">Yassine Chtourou</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-161034" status="INCOMING"> <orgName>Animal Physiology Laboratory, Life Sciences Department</orgName> <desc> <address> <addrLine>UR/08-73, Sfax University, BP 1171, 3000 Sfax</addrLine> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-309870" type="direct"></relation> </listRelation><tutelles><tutelle active="#struct-309870" type="direct"><org type="institution" xml:id="struct-309870" status="INCOMING"> <orgName>Faculty of Sciences</orgName> <desc> <address> <country key="FR"></country> </address> </desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Trabelsi, Khaled" sort="Trabelsi, Khaled" uniqKey="Trabelsi K" first="Khaled" last="Trabelsi">Khaled Trabelsi</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-73827" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13 Place Pasteur, BP 74 1002 Tunis, Belvédère</addrLine><country key="TN"></country></address></desc><listRelation><relation active="#struct-300103" type="direct"></relation><relation active="#struct-301247" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine><country key="TN"></country></address><ref type="url">http://www.pasteur.tn/</ref></desc></org></tutelle><tutelle active="#struct-301247" type="direct"><org type="institution" xml:id="struct-301247" status="VALID"><orgName>Réseau International des Instituts Pasteur</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Fetoui, Hamadi" sort="Fetoui, Hamadi" uniqKey="Fetoui H" first="Hamadi" last="Fetoui">Hamadi Fetoui</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-161034" status="INCOMING"> <orgName>Animal Physiology Laboratory, Life Sciences Department</orgName> <desc> <address> <addrLine>UR/08-73, Sfax University, BP 1171, 3000 Sfax</addrLine> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-309870" type="direct"></relation> </listRelation><tutelles><tutelle active="#struct-309870" type="direct"><org type="institution" xml:id="struct-309870" status="INCOMING"> <orgName>Faculty of Sciences</orgName> <desc> <address> <country key="FR"></country> </address> </desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Mkannez, Ghada" sort="Mkannez, Ghada" uniqKey="Mkannez G" first="Ghada" last="Mkannez">Ghada Mkannez</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-73827" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13 Place Pasteur, BP 74 1002 Tunis, Belvédère</addrLine><country key="TN"></country></address></desc><listRelation><relation active="#struct-300103" type="direct"></relation><relation active="#struct-301247" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine><country key="TN"></country></address><ref type="url">http://www.pasteur.tn/</ref></desc></org></tutelle><tutelle active="#struct-301247" type="direct"><org type="institution" xml:id="struct-301247" status="VALID"><orgName>Réseau International des Instituts Pasteur</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Kallel, Hela" sort="Kallel, Hela" uniqKey="Kallel H" first="Héla" last="Kallel">Héla Kallel</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-73827" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13 Place Pasteur, BP 74 1002 Tunis, Belvédère</addrLine><country key="TN"></country></address></desc><listRelation><relation active="#struct-300103" type="direct"></relation><relation active="#struct-301247" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"><orgName>Institut Pasteur de Tunis</orgName><desc><address><addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine><country key="TN"></country></address><ref type="url">http://www.pasteur.tn/</ref></desc></org></tutelle><tutelle active="#struct-301247" type="direct"><org type="institution" xml:id="struct-301247" status="VALID"><orgName>Réseau International des Instituts Pasteur</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Zeghal, Najiba" sort="Zeghal, Najiba" uniqKey="Zeghal N" first="Najiba" last="Zeghal">Najiba Zeghal</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-161034" status="INCOMING"> <orgName>Animal Physiology Laboratory, Life Sciences Department</orgName> <desc> <address> <addrLine>UR/08-73, Sfax University, BP 1171, 3000 Sfax</addrLine> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-309870" type="direct"></relation> </listRelation><tutelles><tutelle active="#struct-309870" type="direct"><org type="institution" xml:id="struct-309870" status="INCOMING"> <orgName>Faculty of Sciences</orgName> <desc> <address> <country key="FR"></country> </address> </desc></org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author></analytic><idno type="DOI">10.1007/s11064-011-0483-5</idno><series><title level="j">Neurochemical Research</title><idno type="ISSN">0364-3190</idno><imprint><date type="datePub">2011-04-30</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Glutathione</term><term>Manganese</term><term>Neuro-2A cells</term><term>Oxidative stress</term><term>Silymarin</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Manganèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Manganese (Mn) is an essential trace element required for ubiquitous enzymatic reactions. Chronic overexposure to this metal may promote potent neurotoxic effects. The mechanism of Mn toxicity is not well established, but several studies indicate that oxidative stress play major roles in the Mn-induced neurodegenerative processes. Silymarin (SIL) has antioxidant properties and stabilizes intracellular antioxidant defense systems. The aim of this study was to evaluate the toxic effects of MnCl(2) on the mouse neuroblastoma cell lines (Neuro-2A), to characterize the toxic mechanism associated with Mn exposure and to investigate whether SIL could efficiently protect against neurotoxicity induced by Mn. A significant increase in LDH release activity was observed in Neuro-2A cells associated with a significant decrease in cellular viability upon 24 h exposure to MnCl(2) at concentrations of 200 and 800 μM (P < 0.05) when compared with control unexposed cells. In addition, exposure cells to MnCl(2) (200 and 800 μM), increases oxidant biomarkers and alters enzymatic and non enzymatic antioxidant systems. SIL treatment significantly reduced the levels of LDH, nitric oxide, reactive oxygen species and the oxidants/antioxidants balance in Neuro-2A cells as compared to Mn-exposed cells. These results suggested that silymarin is a powerful antioxidant through a mechanism related to its antioxidant activity, able to interfere with radical-mediated cell death. SIL may be useful in diseases known to be aggravated by reactive oxygen species and in the development of novel treatments for neurodegenerative disorders such as Alzheimer or Parkinson diseases.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><country name="Tunisie"><noRegion><name sortKey="Chtourou, Yassine" sort="Chtourou, Yassine" uniqKey="Chtourou Y" first="Yassine" last="Chtourou">Yassine Chtourou</name></noRegion><name sortKey="Fetoui, Hamadi" sort="Fetoui, Hamadi" uniqKey="Fetoui H" first="Hamadi" last="Fetoui">Hamadi Fetoui</name><name sortKey="Kallel, Hela" sort="Kallel, Hela" uniqKey="Kallel H" first="Héla" last="Kallel">Héla Kallel</name><name sortKey="Mkannez, Ghada" sort="Mkannez, Ghada" uniqKey="Mkannez G" first="Ghada" last="Mkannez">Ghada Mkannez</name><name sortKey="Trabelsi, Khaled" sort="Trabelsi, Khaled" uniqKey="Trabelsi K" first="Khaled" last="Trabelsi">Khaled Trabelsi</name><name sortKey="Zeghal, Najiba" sort="Zeghal, Najiba" uniqKey="Zeghal N" first="Najiba" last="Zeghal">Najiba Zeghal</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001484 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001484 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonFranceV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Hal:pasteur-00606405
|texte= Manganese Induces Oxidative Stress, Redox State Unbalance and Disrupts Membrane Bound ATPases on Murine Neuroblastoma Cells In Vitro: Protective Role of Silymarin.
}}
| This area was generated with Dilib version V0.6.29. |  |